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scGPT

✅ Status: ready | Version: whole-human-2024

Overview

Multi-modal transformer (RNA+ATAC+Spatial), attention-based gene interaction modeling

When to choose scGPT

User needs multi-modal analysis (RNA+ATAC or spatial), or explicit attention-based gene interaction maps

Specifications

Property Value
Model scGPT
Version whole-human-2024
Tasks embed, integrate
Modalities RNA, ATAC, Spatial
Species human, mouse
Gene IDs symbol (HGNC)
Embedding Dim 512
GPU Required Yes
Min VRAM 8 GB
Recommended VRAM 16 GB
CPU Fallback Yes
Adapter Status ✅ ready

Quick Start

import omicverse as ov

# 1. Check model spec
info = ov.fm.describe_model("scgpt")

# 2. Profile your data
profile = ov.fm.profile_data("your_data.h5ad")

# 3. Validate compatibility
check = ov.fm.preprocess_validate("your_data.h5ad", "scgpt", "embed")

# 4. Run inference
result = ov.fm.run(
    task="embed",
    model_name="scgpt",
    adata_path="your_data.h5ad",
    output_path="output_scgpt.h5ad",
    device="auto",
)

# 5. Interpret results
metrics = ov.fm.interpret_results("output_scgpt.h5ad", task="embed")

Input Requirements

Requirement Detail
Gene ID scheme symbol (HGNC)
Preprocessing Normalize to 1e4 via sc.pp.normalize_total, then bin into 51 expression bins.
Data format AnnData (.h5ad)
Batch key .obs column for batch integration (optional)

Output Keys

After running ov.fm.run(), results are stored in the AnnData object:

Key Location Description
X_scGPT adata.obsm Cell embeddings (512-dim)
scgpt_pred adata.obs Predicted cell type labels 
import scanpy as sc

adata = sc.read_h5ad("output_scgpt.h5ad")
embeddings = adata.obsm["X_scGPT"]  # shape: (n_cells, 512)

# Downstream analysis
sc.pp.neighbors(adata, use_rep="X_scGPT")
sc.tl.umap(adata)
sc.tl.leiden(adata, resolution=0.5)
sc.pl.umap(adata, color=["leiden"])

Resources

Hands-On Tutorial

For a step-by-step walkthrough with code, see the scGPT Tutorial Notebook.